In his early work on the Citric Acid Cycle, Hans Krebs made measurements of oxygen consumption...

In his early work on the Citric Acid Cycle, Hans Krebs made measurements of oxygen consumption by suspensions of muscle cells. He found that addition of succinate, fumarate or malate to the cell suspension stimulated oxygen consumption, and that the amount of oxygen consumed was about 10-fold more than the amount needed for the complete oxidation of the added compound. Moreover, the succinate, fumarate or malate was not consumed by the cell suspension but was still detectable at the end of the experiment. What is the correct interpretation of these observations?

Expert Solution

ANSWER:-

In TCA cycle succinate, fumarate or malate is the intermediate compound.
Apart form that muscle suspension also contains product of dlycolysis (pyruvate) which is the first substrate of TCA cycle when it react with acetyl CoA and convert into citrate.
This citrate molecule further converted into isocitrate and alpha-keto glutarate.
During the conversion of isocitrate to alpha-keto glutarate oxygen is consumed and CO2 is released.
Thats why oxygen consumption is more.

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