In: Biology
Answer- e. Telomere-ARS-centromere-100,000 bp insert- telomere
Explanation-
1. The yeast artificial chromosome (YAC) is capable of replicate in bacteria and yeast as host and carries large DNA insert from 100kb to 10Mb.
2. The structural elements of YAC are -
TEL - Marker - ARS - CEN -EcoRI site - Marker - TEL
TEL - this telomeric sequences are separated by BamHI site and after treatment with BamHI , circular plasmid converted into linear
ARS - it is origin of replication
CEN - it is required for stability of YAC during mitosis
Marker - generally antibiotic resistance gene is used as marker so that cells can be identified when grown in presence of particular antibiotic
EcoRI site- this site is use to cut plasmid and to create cohesive ends at which DNA can be inserted
BamHI - this site is use to linearize the circular plasmid
So the yeast artificial chromosome (YAC) that most likely to segregate correctly is Telomere-ARS-Centromere-100,000 bp insert- telomere.