Which of the combinations represents a complete yeast artificial chromosome (YAC) that is most likely to...

Which of the combinations represents a complete yeast artificial chromosome (YAC) that is most likely to segregate correctly? Explain your reasonings thoroughly!
1. telomere-ARS-centromere
2. telomere-ARS-centromere-100,000 bp insert
3. telomere-centromere-100,000 bp insert-telomere
4. telomere-ARS-centromere-11,000 bp insert-telomere suitable selectable markers
5. telomere-ARS-centromere-100,000 bp insert-telomere

Solutions

Expert Solution

Answer- e. Telomere-ARS-centromere-100,000 bp insert- telomere

Explanation-

1. The yeast artificial chromosome (YAC) is capable of replicate in bacteria and yeast as host and carries large DNA insert from 100kb to 10Mb.

2. The structural elements of YAC are -

TEL - Marker - ARS - CEN -EcoRI site - Marker - TEL

TEL - this telomeric sequences are separated by BamHI site and after treatment with BamHI , circular plasmid converted into linear

ARS - it is origin of replication

CEN - it is required for stability of YAC during mitosis

Marker - generally antibiotic resistance gene is used as marker so that cells can be identified when grown in presence of particular antibiotic

EcoRI site- this site is use to cut plasmid and to create cohesive ends at which DNA can be inserted

BamHI - this site is use to linearize the circular plasmid

So the yeast artificial chromosome (YAC) that most likely to segregate correctly is Telomere-ARS-Centromere-100,000 bp insert- telomere.

gladiator answered 1 year ago

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