Question

Explain Michaelis-Menten plots and demonstrate how to calculate Km, kcat, and catalytic efficiency.

Answer 1

Michaelis menten plot is one of the best model of enzyme kinetics. Enzymes bind to substrate and further it converted the substrate into a product. This reaction can be represented as given below:

The Michaelis menten equation can be represented as:

In this question Vmax depicts the maximum velocity, [S] depicts the concentration of substrate and Km represent the Michaelis constant. Km is the concentration of substrate at 50% of maximum velocity.

The Michaelis menten plot can be represented as given below where it is plotted against substrate concentration v/s relative velocity.

Km , Kcat and catalytic efficiency can be calculated by following formulas:

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