Question

What kind of reaction occurs when anti-tTG is bind to the tissue transglutaminase? What kind of biosensor can measure their reaction? How will the measurement be made for their reaction?

Answer 1

Auto immune reaction that is Type III hypersensitivity reaction occurs when anti tTG bind to the tissue transglutaminase .This is a type of auto immune disorder because here antibodies that are made in response to cereal gluten protein act against body'own enzyme that is transglutaminase. These anti tTG form immune complexes with transglutaminase and leads to celic disease.

These anti tTG-transglutaminase complex form the diagnostic test basis for celic disease.

The best biosensor used to measure their reaction are Electrical affinity biosensor that are based on specific antigen-antibody reaction and called as immunosensors.In immunosensors the antigen that is transaminase is immobilised on interface surface (electrode) and then serum of patient is added if antitTG are formed in this patient then these antibodies bind with transglutaaminase enzyme and form immune complex then labeled secondary Ab is added that is bind to Anti t TG antibody .the label used for 2° Ab may be enzyme which give specific reaction with addition of its substrate or nanoparticle that give redox reaction and generation of  electrochemical or amperometric signal due to These signals are detected and intensity of signal measures the transglutaminase-antitTG immune complex reaction .