Expression vectors ______. a. require a promotor if used in yeast but not in bacteria. b....

Expression vectors ______.

a. require a promotor if used in yeast but not in bacteria.

b. are created by oligonucleotide-directed mutagenesis.

c. are only used in bacteria and yeast.

d. can allow for production of fusion proteins.

Solutions

Expert Solution

Answer: d. can allow for production of fusion proteins.

Expression vectors must have expression signals such as strong promoters and are used in bacteria, yeast, baculovirus, plant, mammalian and cell-free systems (prokaryotes & eukaryotes as well). These are used for site-directed mutagenesis. prokaryotes expression vectors would have a Shine-Dalgarno sequence, while eukaryotes expression vectors would contain the Kozak consensus sequence. vectors are generally designed for cloning a foreign gene into a host genome to produce proteins which are not produced by host. Fusion of one gene to another gene will produce a fusion protein. For example pGEX-TT expression vector used for production of GST fusion proteins, it Contains the polylinker from pEG-KT, tac promoter, lacIq repressor, ampicillin resistance.

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