Expression vectors ______. a. require a promotor if used in yeast but not in bacteria. b....

Expression vectors ______.

a. require a promotor if used in yeast but not in bacteria.

b. are created by oligonucleotide-directed mutagenesis.

c. are only used in bacteria and yeast.

d. can allow for production of fusion proteins.

Expert Solution

Answer: d. can allow for production of fusion proteins.

Expression vectors must have expression signals such as strong promoters and are used in bacteria, yeast, baculovirus, plant, mammalian and cell-free systems (prokaryotes & eukaryotes as well). These are used for site-directed mutagenesis. prokaryotes expression vectors would have a Shine-Dalgarno sequence, while eukaryotes expression vectors would contain the Kozak consensus sequence. vectors are generally designed for cloning a foreign gene into a host genome to produce proteins which are not produced by host. Fusion of one gene to another gene will produce a fusion protein. For example pGEX-TT expression vector used for production of GST fusion proteins, it Contains the polylinker from pEG-KT, tac promoter, lacIq repressor, ampicillin resistance.

Hope this helped you!

Thank You So Much! Please Rate this answer as you wish.("Thumbs Up")

queen_honey_blossom answered 2 years ago

Bacteria and other kinds of pathogens (like the yeast Candida auris) used to be very susceptible...

Bacteria and other kinds of pathogens (like the yeast Candida auris) used to be very susceptible (likely to be harmed by) to drugs used to kill them. However, today, we find that many pathogens that make people sick are resistant (unlikely to be harmed by) to the same drugs we used to use. Explain, in your own words, where these multi-drug resistant pathogens (superbugs) come from.

Describe plasmid vectors and procedures used for high-level inducible expression and affinity purification of recombinant proteins...

Describe plasmid vectors and procedures used for high-level inducible expression and affinity purification of recombinant proteins using E.coli.

List 3 similarities in how DNA is isolated from bacteria and from yeast.

Three isoforms of haemoglobin are needed to be expressed and purified using the budding yeast expression...

Three isoforms of haemoglobin are needed to be expressed and purified using the budding yeast expression system. These haemoglobins are very similar both in size and amino acid sequence. Briefly describe how would you design your experiments to express and purify these proteins to homogeneity.

1.Carry out library search to find out if the bacteria and yeast form alcohol in the...

1.Carry out library search to find out if the bacteria and yeast form alcohol in the absence of oxygen or they possess a metabolic pathway that does not involve oxygen. Write down your findings 2. What would happen if the first enzyme in glycolysis is irreversible inhibited by a toxic substance? PLEASE HELP ME ALL QUESTIONS

A) Create two vectors in R3 ( 3D vectors) such that they are orthogonal. b) Using...

A) Create two vectors in R3 ( 3D vectors) such that they are orthogonal. b) Using the two vectors from a) above, determine the cross product of the two vectors c)Is it possible to write the vector [0,0,1] using scalar multiples of these vectors?

Three vectors A, B and C are related via A+B=C. Which of the following diagrams illustrates these vectors?

Three vectors A, B and C are related via B+C = A. Which of the following diagrams illustrates these vectors?

Compare and contrast how the initiation complex is assembled in bacteria (E. coli) and in eukaryotes (budding yeast cells).

Compare and contrast how the initiation complex is assembled in bacteria (E. coli) and in eukaryotes (budding yeast cells). How do the bacterial and budding yeast ribosomes determine which start codon to initiate translation? Include in your description a discussion of the cis-acting elements, trans-acting factors, and energy requirements required for building the initiation complex in each system. The best response for this question will NOT BE A LIST of the requirements. It will clearly COMPARE and CONTRAST this process...

OPEN QUESTIONS: You are researching mechanisms involved in wdf1 gene expression regulation in yeast 1. Please...

OPEN QUESTIONS: You are researching mechanisms involved in wdf1 gene expression regulation in yeast 1. Please shortly describe how to identify enhancers regulating expression of the wdf1 gene. 2. You have discovered that Wou1 protein is the transcriptional factor that is involved in wdf1 activation. You next goal is to identify other factors, that are interacting with Wou1 protein and are required for wdf1 activation. Please shortly describe experimental approaches to achieve this goal. 3. You have discovered that Wou1...

Question