Question

In: Biology

List 3 similarities in how DNA is isolated from bacteria and from yeast.

List 3 similarities in how DNA is isolated from bacteria and from yeast.

Solutions

Expert Solution

The first step in both the processes is the removal of cell wall. The cell wall is made up of peptidoglycan in bacteria and of glucan and mannons in yeast. The cell wall is broken down in both the cases.

II similarity is that won the cell wall is removed, plasma membrane is broken down in both the cells. Plasma membrane breakdown is important for the exposure of cytoplasmic content in both the cells.

The last step is also common in both the cells because it involves precipitation of DNA molecules using chilled ethanol. DNA is present in solubilzed form in aqueous phase and is precipitated out by the addition of chilled ethanol.

(Note - differences in the isolation of DNA from cell occur because different cells have different structure and composition, but the principal for the extraction of DNA remain the same for the types of cells).

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