1. Data and experimental conceptual questions: a. Define
replication and randomization b. Why would a researcher care about
randomization? c. Define blocking and give an example of an
experiment that would require this. d. Define accuracy and
precision.
Why is it important that the initiation of DNA replication is
carefully regulated? What is the proposed role of DnaA in this
process?
What extra proteins are necessary for lagging-strand synthesis
that are not needed in leading-strand synthesis?
Replication in eukaryotes is similar to that of prokaryotes.
However initiation is different. Describe how initiation of
replication occurs and how it is tied to the cell cycle.
Explain in general terms how DNA replication can occur in a
chromatin environment and...
1. The use of RNA primer in DNA replication increases the
fidelity of DNA replication. Explain this statement.
2. Some errors in DNA replication can escape repair and this
is to some extent is beneficial for the evolution. Explain
why?
1. A) Why is it important to control illumination for live
cells? B) Briefly explain how live cell imaging chambers
can be used to control four parameters (name and indicate
importance) that contribute to maintaining healthy cells.
Explain in detail (use a drawing to support you explanation),
why after each round of replication, the new daughter strand is
~20nucleotide’s shorter than the parent strand.
Explain why the four steps involved in the control function are
important. How are the various ratios used by accountants and
managers differently in the control function?
a. Explain how eukaryotic chromosomes become shortened after
each round of replication.
b. Why does this not happen in prokaryotes?
c. How does the cell attempt to remedy this problem