Question

In: Statistics and Probability

3) An experiment was performed where HEK 293 cells expressing TLR5 was treated with flagellin and...

3) An experiment was performed where HEK 293 cells expressing TLR5 was treated with flagellin and the following absorbance readings were measured at 655 nm.

No treatment: 0.232, 0.247, 0.239

Flagellin treatment: 0.355, 0.377, 0.350

What is the P-value as calculated by T-test? Assume 2-tails and equal variance among the groups.

Solutions

Expert Solution

Ho :   µ1 - µ2 =   0                  
Ha :   µ1-µ2 ╪   0                  
                          
  
Sample #1   ---->   1                  
mean of sample 1,    x̅1=   0.239                  
standard deviation of sample 1,   s1 =    0.0075                  
size of sample 1,    n1=   3                  
                          
Sample #2   ---->   2                  
mean of sample 2,    x̅2=   0.361                  
standard deviation of sample 2,   s2 =    0.0144                  
size of sample 2,    n2=   3                  
                          
difference in sample means =    x̅1-x̅2 =    0.2393   -   0.4   =   -0.121  
                          
pooled std dev , Sp=   √([(n1 - 1)s1² + (n2 - 1)s2²]/(n1+n2-2)) =    0.0115                  
std error , SE =    Sp*√(1/n1+1/n2) =    0.0094                  
                          
t-statistic = ((x̅1-x̅2)-µd)/SE = (   -0.1213   -   0   ) /    0.01   =   -12.97
                          
Degree of freedom, DF=   n1+n2-2 =    4                  
p-value =        0.0002   (excel function: =T.DIST.2T(t stat,df) )              

orchestra answered 2 years ago
Next > < Previous

Related Solutions

We performed an experiment where first we prepared a buffer with a pH of about 5...
We performed an experiment where first we prepared a buffer with a pH of about 5 by adding a specific amount of sodium acetate and acetic acid and then prepared another buffer with the same pH by dissolving sodium acetate in water and adding HCl until the pH was 5. Then when we slowly added HCl/NaOH to half of each solution we calculated that the buffer capacity of the second solution was slightly better. Why might that be? Or should...
We performed a final experiment where we determined the tightness of DNA packaging for this gene...
We performed a final experiment where we determined the tightness of DNA packaging for this gene in wild-type toe cells, and in toe cells in which the Talon enhancer had been mutated as described for constructs g-k. In this experiment we isolated chromatin and determined if an enzyme cuts in the gene very well or very poorly, depending on how tightly packed the DNA is at the site of cutting. c. Suggest why the ability of an enzyme to cut...
Question 3 A brain specialist performed an experiment to see if sensory deprivation over an extended...
Question 3 A brain specialist performed an experiment to see if sensory deprivation over an extended period of time reduces the alpha-wave frequencies produced by the brain. To determine this, 10 subjects, inmates of a Correctional Centre, were randomly selected. First, the members of the group were allowed to remain in their cells. Seven days later, alpha-wave frequencies were measured in Hertz for all subjects (nonconfined). After that, the same group of subjects were placed in solitary confinement for seven...
in an experiment i performed 3 EDTA complexometric tritrations to determine the amount of Al3+, Ca2+...
in an experiment i performed 3 EDTA complexometric tritrations to determine the amount of Al3+, Ca2+ and Mg2+ in 0.6499 g of antacid powder. i made my sample for titration following the method below. A sample of antacid powder ("Hardy's indigestion powder") is supplied containing aluminium hydroxide, calcium carbonate and magnesium carbonate. Record this accurate mass. Add 5 mL of water and then 10 mL of 3 mol L-1 HCl Heat the mixture (microwave for 30 seconds) to boiling and...
Question 3 A brain specialist performed an experiment to see if sensory deprivation over an extended...
Question 3 A brain specialist performed an experiment to see if sensory deprivation over an extended period of time reduces the alpha-wave frequencies produced by the brain. To determine this, 10 subjects, inmates of a Correctional Centre, were randomly selected. First, the members of the group were allowed to remain in their cells. Seven days later, alpha-wave frequencies were measured in Hertz for all subjects (nonconfined). After that, the same group of subjects were placed in solitary confinement for seven...
Question 3 A brain specialist performed an experiment to see if sensory deprivation over an extended...
Question 3 A brain specialist performed an experiment to see if sensory deprivation over an extended period of time reduces the alpha-wave frequencies produced by the brain. To determine this, 10 subjects, inmates of a Correctional Centre, were randomly selected. First, the members of the group were allowed to remain in their cells. Seven days later, alpha-wave frequencies were measured in Hertz for all subjects (nonconfined). After that, the same group of subjects were placed in solitary confinement for seven...
3) The following “cycle of copper” experiment is performed in some general chemistry laboratories. The series...
3) The following “cycle of copper” experiment is performed in some general chemistry laboratories. The series of reactions starts with copper wire and ends with metallic copper. The steps are as follows: I. A piece of copper wire of known mass is allowed to react with concentrated nitric acid, the products are copper(II) nitrate , nitrogen dioxide, and water. II. The copper(II) nitrate is treated with a sodium hydroxide solution to form copper(II) hydroxide. III. On heating, the copper(II) hydroxide...
If you want to reproduce the shuttling experiment as performed during week 3. you will need...
If you want to reproduce the shuttling experiment as performed during week 3. you will need to purchase Cycloheximide (CHX) from one of three potential suppliers. CHX has a molar mass of 281.35 g/mol and can be dissolved in DMSO, water, or EtOH but with different solubilities. You will need to use CHX at a final working concentration of 17 μg/mL in a 150 mm dish with a total media volume of 14 mL. Determine how much of the stock...
In an experiment, where we need to isolate lipids from horse red blood cells, one of...
In an experiment, where we need to isolate lipids from horse red blood cells, one of the steps is too "Add 100µL of choloform;methanol (9:1) and mix vigorously to dissolve the membrane lipids. " What happens in this step, what would happen if you added water instead of chloroform/methanol?
. An experiment was performed on a certain metal to determine if the strength is a...
. An experiment was performed on a certain metal to determine if the strength is a function of heating time (hours). Results based on 20 metal sheets are given below. Use the simple linear regression model. ∑X = 40 ∑X2 = 200 ∑Y =   80 ∑Y2 = 1120 ∑XY = 460 Find the estimated y intercept and slope and write the equation of the least squares regression line. Estimate Y when X is equal to 3 hours. Also determine the...
ADVERTISEMENT
Subjects
ADVERTISEMENT
Latest Questions
ADVERTISEMENT