In an experiment, where we need to isolate lipids from horse red blood cells, one of...

In an experiment, where we need to isolate lipids from horse red blood cells, one of the steps is too "Add 100µL of choloform;methanol (9:1) and mix vigorously to dissolve the membrane lipids. "

What happens in this step, what would happen if you added water instead of chloroform/methanol?

Expert Solution

hope it's clear to you now, if not please mention in comment. I'll help you out

gladiator answered 2 years ago

In an experiment to isolate the lipids from horse red blood cells, one of the steps...

In an experiment to isolate the lipids from horse red blood cells, one of the steps is too "Add 500µL of 10mM sodium phosphate buffer (pH. 7.0). Resuspend the pellet thoroughly by vortexing." What happens in this step of the lipid isolation period? (hint: the supernatant in the next centrifuge step will be red)

The cell membranes of mammalian red blood cells are permeable to urea. If red blood cells...

The cell membranes of mammalian red blood cells are permeable to urea. If red blood cells are dropped into a solution of urea that is identical in osmotic pressure (isosmotic) to the cytoplasm of the cells, although the cells do not swell and burst as quickly as when they are dropped simply into pure water, they eventually swell and burst. Explain. Also discuss how you would design a solution into which red cells could be placed without ever swelling. (Hint:...

The following experiment is carried out in a physiology lab. Red blood cells are placed in...

The following experiment is carried out in a physiology lab. Red blood cells are placed in different solutions: distilled water, 0.45% saline, 0.9% saline, 5% urea, and 5% glucose. The molecular mass for each molecule is given: NaCl = 58.44 gm/mole; urea = 60.06 gm/mole; glucose = 180.16 gm/mole. For each of the solutions used, calculate the osmolarity. To do this, you will need to convert solutions given in percents to osmolarity. A 0.45% saline solution has 0.45 gm/100 mL...

Osmosis and Tonicity experiment: Red blood cells prefer an isotonic (isosmotic) environment. What happens to red...

Osmosis and Tonicity experiment: Red blood cells prefer an isotonic (isosmotic) environment. What happens to red blood cells place into a hypertonic (hyperosmotic) environment? Why is that bad for a patient? What happens to red blood cells placed into a hypotonic (hypoosmotic) environment? Why is that bad for a patient?

Which of the following describes the production of red blood cells? Select one: a. The kidneys...

Which of the following describes the production of red blood cells? Select one: a. The kidneys release erythropoietin which stimulates RBC production. b. High oxygen levels stimulate the production of more red blood cells. c. RBC production occurs in the lymph nodes. d. The production of angiotensinogen is an intermediate step in making more red blood cells. e. more than one answer is correct Which of the following does NOT increase venous return to the heart? Select one: a. skeletal...

Describe the origin and lifecycle of the red blood cells.

Mr. X collected blood samples from different subjects and he is planning to isolate CD4+ T-cells...

Mr. X collected blood samples from different subjects and he is planning to isolate CD4+ T-cells only. After the isolation of CD4+ T-cells he wants to extract protein and study the expression of CD4 (which is a glycoprotein) among the subjects. Based on these, answer the following: a. What technique should Mr. X use to isolate CD4+ T-cells from other blood cells? Give a brief description for this technique b. What technique should Mr. Xuse to separate the protein he...

1. Which of these cells is capable of phagocytosis? Red blood cells NK cells Mast cells...

1. Which of these cells is capable of phagocytosis? Red blood cells NK cells Mast cells Dendritic cells 2. Which of these chemicals aids in inducing apoptosis in a virally-infected cell? Histamine Eicosanoids Heparin Granzyme 3. Which of these cell types is capable of producing cytotoxic chemicals against a multicellular parasite? Dendritic cells Mast cells Eosinophils

Someone with AB+ blood has red blood cells with the A, B and Rh _________ on...

Someone with AB+ blood has red blood cells with the A, B and Rh _________ on the surface of their red blood cells. They do not have any__________ circulating in their plasma. They are the universal recipient because they can receive any blood type in a transfusion without the danger of a __________ reaction.

Thalassemia is a genetic disorder where affected individuals have red blood cells that produce too much...

Thalassemia is a genetic disorder where affected individuals have red blood cells that produce too much iron. This disease is inherited as an autosomal recessive (tt). However, heterozygous individuals (carriers= Tt) may have some immunity to malaria (similar to sickle cell anaemia). Human blood samples are obtained from a Mediterranean population of 100 people where malaria is endemic. Of these, 50 people are found to be carriers (Tt), and additional 3 people have thalassemia (tt). Use these observed results to...

Question