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Question 1 What do the following terms mean with respect to RNAi in C. elegans: It...

Question 1

What do the following terms mean with respect to RNAi in C. elegans:

  • It is highly specific
  • It is highly potent
  • It is heritable

Question 2

Why should we analyze the RNAi phenotype in F1 generation? Discuss the worm husbandry involved to make sure the RNAi phenotype can be scored in F1 generation.

Solutions

Expert Solution

RNAi means interferance RNA & C .ELEGANS means caenorabditis elegans

RNA interferance is a widely used technique in which double stranded DNA is introduced into an organism resulting in gene silencing.

, RNA interference (RNAi) has become an important tool for C. elegans research and the scientific community in general. RNAi is a potent, specific, rapid, and simple method for determining loss-of-function phenotypes of genes in C. elegans, and has been the basis of several genome-wide screens.

Elegans has made this species a powerful model genetic organism. Since its discovery, RNA interference (RNAi) has become an important tool for C. elegans research and the scientific community in general. RNAi is a potent, specific, rapid, and simple method for determining loss-of-function phenotypes of genes in C.elegans

gladiator answered 2 years ago
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