Question

What is the correct order of the utilization of the following enzymes during synthesis of an Okazaki fragment? 1. RNA Primase 2. DNA Polymerase III 3. DNA Ligase 4. DNA Helicase 5. DNA Polymerase I

Answer 1

The answer is

4. DNA
1. RNA Primase
2. DNA Polymerase III
5. DNA Polymerase I
3. DNA Ligase

Find the below explanation, how they work together...

4. DNA Helicase
It unwinds the Double helix of the DNA and allow for its replication.
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1. RNA Primase
After unzipping, A DNA Dependent RNA synthesis enzyme similar to RNA polymease names RNA primase or Primase, synthesizes a small strectch of starter RNA molecule which is called primer.
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2. DNA Polymerase III
The main replication enyzmes, it adds the nucleotides corresponding to the template strand to 3' end of the RNA primer by using its 5'>3' polymarization activity.
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5. DNA Polymerase I
It is a repair enzyme, it has its unique property i.e. 5'>3' exonuclease activity, due to this activity it binds to RNA primers and replace those Ribonucleotides with corresponding deoxy ribonucleotides.
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3. DNA Ligase
After DNA polymease I activity, the DNA ligase binds the all discountinous synthesized fragments to synthesis a complete daughter strand on lagging strand.