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A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine,...

A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, t c , was found to be 201.4 s with a baseline peak width, w c , of 13.8 s. The retention time for aspartame, t a , was 265.0 s with a baseline peak width, w a , of 21.4 s. The retention time for the unretained solvent methanol was 40.9 s.

Calculate the average plate height, H , in micrometers for this separation, given that it was performed on a 25.2 cm long column.

Calculate the resolution, R , for this separation using the widths of the peaks.

Calculate the resolution if the number of theoretical plates were to increase by a factor of 1.5

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queen_honey_blossom answered 2 years ago
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