The chromatographic separation happens in a specific way. Answer the following questions about the procedure: We...

The chromatographic separation happens in a specific way. Answer the following questions about the procedure:

We fill the column approximately 2/3 of the way. What would be the consequence of only barely filling the column with alumina?

We gradually add petroleum ether to the column, always making sure to have the petroleum ether level adjust to right above the alumina level, before adding more. Why is this necessary?

What would be the specific consequence of eluting the column with diethyl ether first, and then petroleum ether second?

Expert Solution

Q: We fill the column approximately 2/3 of the way. What would be the consequence of only barely filling the column with alumina?

Ans: The separation would not happen or would not be complete because the longer the column, better the separation

Q: We gradually add petroleum ether to the column, always making sure to have the petroleum ether level adjust to right above the alumina level, before adding more. Why is this necessary?

Ans: if solvent not above the aluminium level column will be dry, there will be gaps in column, separation not properly happens.

Q: What would be the specific consequence of eluting the column with diethyl ether first, and then petroleum ether second?

Ans: polarity of diethyl ether more compared to petroleum ether, therefore any impurity present it will be washed out, after when run petrolium ether the total diethyl eter washed out, column becomes good for separation.

Amanda K answered 2 years ago

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