Question

Essay. Write these in essay format, that will allow me to better assign partial credit.

Meselson and Stahl conducted an experiment on DNA replication. Describe the experiment in your own words. What was their question (5 pts)? What were their methods (10 pts)? What were the results (6 pts)? What did they conclude (5 pts)?

Answer 1

SEMI-CONSERVATIVE METHOD OF DNA REPLICATION

This was tested by Mathew Melson and Franklin Stahl in 1958. They cultured E.Coli bacteria in a culture medium containing heavy isotopes o niitrogen (15N). After several generation these cultured DNA transferred to a normal medium containg 14N isotope of nitrogen. from this newly formed strand have one heavier and another one is lighter strand, thatmeans 50% N14-N 15 type. that shows that one replication strand is conserved in the daughter and other strand is newly synthesised.

Interpretting the result

After replication generation I has one heavy strand which received from the parent ang light strand is newly synthesised.

When this hybrid DNA is replicated in second generation shows 50% chance of semi conservative replication.