Question

In: Biology

1. You are given two Neisseria gonorrhoeae isolates and are told that one can produce a...

1. You are given two Neisseria gonorrhoeae isolates and are told that one can produce a β-lactamase and that the other one cannot. You can’t find any Nitrocefin Dry Slides in the lab but you have access to the antimicrobial disks used in Kirby-Bauer tests. Which antimicrobial drug would you use to determine if β-lactamase is being produced?

A) streptomycin

B) chloramphenicol

C)ampicillin

D)kanamycin

2. The results of an MIC tube dilution test can be used to determine whether an antibiotic is...

A) bacteriostatic

B) bactericidal

C) bactericidal and bacteriostatic

D) none

3. What is the purpose of nitrocefin in the beta-lactamase test?

A) Nitrocefin is a chromogenic compound that cleaves beta-lactam rings.

B) Nitrocefin is a chromogenic compound that tests if a drug has a beta-lactam ring.

C) Nitrocefin is a chromogenic compound that acts as a target for beta-lactamases.

D) Nitrocefin is a chromogenic compound that inhibits the activity of beta-lactamases.

Expert Solution

1.
c) Ampicillin

Ampicillin is an antibiotic that contains the Beta-lactam ring, which can be used to determine if the beta-lactamase enzyme is being produced.

2.
B) bactericidal

An MIC tube dilution test is used to determine the concentration of the agent at which the bacterial culture would not grow at all. This is a measure of Bactericidal agents.

3.
C) Nitrocefin is a chromogenic compound that acts as a target for beta-lactamases.

Nitrocefin is a compound that produces a colored product when cleaved by an Beta-Lactamase. This property allows for the visual detection of the presence of Beta-lactamases in a solution.

gladiator answered 2 years ago

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