E. coli is a mesophile that
grows best at 37 degrees Celsius in neutral pH environments.
E. coli is a facultative aerobe
and is able to grow without oxygen, but it can
extract more energy from its nutrient source and
grow faster if oxygen is present.
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Growing E. coli is easy and fast
Scientists first chose to work with E. coli because it
was easy and fast to grow in the laboratory.
There are several features of E. coli that make it easy
to culture:
- It likes it warm – but not too warm.Because E. coli is
a gut bacterium, it grows best at body temperature (37.4ºC). This
is an easy temperature for scientists to work with in the
laboratory.
- It isn’t fussy about nutrition. E. coli can obtain
energy from a wide variety of sources. In its natural environment
(the gut), it consumes digested foodstuffs. In a laboratory
context, E. coli can be fed easily and cheaply – think
chicken soup for bacteria
- It can grow with or without oxygen. In the gut, E.
coli grows anaerobically (in the absence of oxygen). However,
unlike some anaerobic bacteriaE. coli also grows well in
aerobicenvironments, such as a culture flask in a laboratory.
- It grows fast. Under ideal conditions, individual E.
colicells can double every 20 minutes. At that rate, it would
be possible to produce a million E. coli cells from one
parent cellwithin about 7 hours. Fast growth means that experiments
involving E. coli can be done quickly, conveniently and
cheaply.
In its normal habitat of the intestinal tract,
E. coli is beneficial. The bacteria participate in the digestion of
food, and produce vitamin K and B—complex vitamins