Question

In: Biology

Researchers grew populations of identical Escherichia coli (E. coli) bacteria in a growth medium that contained...

Researchers grew populations of identical Escherichia coli (E. coli) bacteria in a growth medium that contained a low concentration of glucose and a high concentration of citrate, a substance that is not typically consumed by E. coli. For thousands of generations, the bacteria used only glucose as an energy source and grew relatively slowly (and to a low density because of the low concentration of glucose). After about 30,000 generations, one population emerged that began to rapidly grow to a much higher density. The researchers hypothesized that the bacteria evolved the ability to use citrate as an energy source and referred to them as + Cit . To test the hypothesis, the researchers grew separate populations of the + Cit bacteria and bacteria from the original population ( − Cit ) in a growth medium that contained only citrate.

(a)   Describe one outcome that would demonstrate that a given population has evolved (think microevolution).

(b)   Identify the dependent variable measured in the experiments described above.

(c)   Predict the results obtained by the researchers when they grew the + Cit and − Cit bacteria in the medium that contained only citrate.

(d)   The researchers claim that the + Cit mutation increases the fitness of the bacteria. Provide reasoning to support the claim.

Solutions

Expert Solution

(PLEASE UP VOTE THANK YOU......)


Related Solutions

Write three to five sentences about Escherichia Coli bacteria. Such as the shape of the bacteria,...
Write three to five sentences about Escherichia Coli bacteria. Such as the shape of the bacteria, gram stain, diseases that can be caused by this bacteria, and etc.
The bacterium Escherichia coli (or E. coli) is a single-celledorganism that lives in the gut...
The bacterium Escherichia coli (or E. coli) is a single-celled organism that lives in the gut of healthy humans and animals. When grown in a uniform medium rich in salts and amino acids, these bacteria swim along zig-zag paths at a constant speed of 20 μm/s. The figure shows the trajectory of an E. coli as it moves from point A to point E. Each segment of the motion can be identified by two letters, such as segment BC. For the...
Briefly describe the Escherichia coli (E. coli) lac operon and its regulation.
Briefly describe the Escherichia coli (E. coli) lac operon and its regulation.
Escherichia coli O157:H7 is a pathogenic strain of E. coli associated with consumption of raw or...
Escherichia coli O157:H7 is a pathogenic strain of E. coli associated with consumption of raw or undercooked foods. When exposed to temperatures above its normal growth temperature (temperatures that do not kill it), it may change its membrane lipid composition to counteract the rise in temperature. Which of the following changes would be most likely? Select one: a. A greater proportion of the unsaturated 16-carbon palmitoleate b. A greater proportion of the saturated 12-carbon laurate c. Generating a thinner membrane...
Escherichia coli fully labelled with 15N is allowed to grow in 14N medium. The two strands...
Escherichia coli fully labelled with 15N is allowed to grow in 14N medium. The two strands of DNA molecule of the first generation bacteria have (a) different density and do not resemble parent DNA (b) different density but resemble parent DNA (c) same density and resemble parent DNA (d) same density but do not resemble parent DNA.
Part A Escherichia coli (E. coli) can under optimal conditions double every 20.0 minutes. How long...
Part A Escherichia coli (E. coli) can under optimal conditions double every 20.0 minutes. How long would it take a single cell to saturate a 5.0 L culture assuming that optimal growth conditions prevaled and that no cells died? The maximum cell denisty in a saturated culture is 1010 cells mL-1. Enter your answer to the nearest tenth of an hour. Part B Consider the information in Part A and decide how long it would take for the 5.0 L...
Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The...
Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The recombinant protein can be considered a product of cell culture even though it is not secreted from the cells; it is synthesized in addition to normal E. coli biomass. Ammonia is used as the nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula of CH1.55O0.31N0.25. The yield of biomass (excluding recombinant protein) from glucose is measured as 0.48 g...
Given a recent outbreak of illness caused by E. coli bacteria, the mayor in a large...
Given a recent outbreak of illness caused by E. coli bacteria, the mayor in a large city is concerned that some of his restaurant inspectors are not consistent with their evaluations of a restaurant’s cleanliness. In order to investigate this possibility, the mayor has five restaurant inspectors grade (scale of 0 to 100) the cleanliness of three restaurants. The results are shown in the accompanying table. (You may find it useful to reference the q table.) Inspector Restaurant 1 2...
Wild-type E. coli can grow on minimal medium. You have isolated a mutant strain of E....
Wild-type E. coli can grow on minimal medium. You have isolated a mutant strain of E. coli that grows poorly in minimal medium that contains lactose and arabinose and lacks glucose. In ONPG-containing minimal medium lacking glucose the mutant strain produces very low levels of b-galactosidase activity in both the absence and presence of IPTG. The strain does not grow well when transformed with a plasmid containing the lacZ and lacY genes. The strain also does not grow well when...
E. coli cells are growing in a medium containing lactose but no glucose. Indicate whether each...
E. coli cells are growing in a medium containing lactose but no glucose. Indicate whether each of the following changes or conditions would increase, decrease, or not change the expression of the lac operon. For full credit, you must explain your answer. It may be helpful to draw a model depicting what is happening in each situation. (a) Addition of a high concentration of glucose (b) A mutation that prevents dissociation of the Lac repressor from the operator (c) A...
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT