Question

mechanical method disruption and chemical method disruption, which method is suitable to recover the intracellular Ectoine at industrial scale by providing scientific justification

Answer 1

As you know, cell disruption is a process of extraction of intracellular fluid from the cell by opening the cell wall through different methods, such as mechanical methods and chemical methods.

Here the question was about what method is suitable for recovering the intracellular ectoine at an industrial scale production.

ECTOINE is a natural compound found in the intracellular fluids of several species of bacteria.

The extraction of ectoine by chemical method for commercial production is time-consuming and very expensive, follows less availability of raw materials such as enzymes, solvents etc....

considering the mechanical method, it is more suitable for the extraction of ECTOINE for commercial purposes because it includes cheap production cost and higher the frequency of production.

mechanical methods of cell disruption

1. mortar and pastel ( grinding)
2. blending
3. bead beating
4. ultrasonication
5. homogenization

chemical methods of cell disruption

1. using detergents
2. using enzymes such as lysozyme
3. using solvents (organic/inorganic)
4. using osmotic shock

Considering the accuracy of production, the chemical method is more suitable than the mechanical method.

therefore from the above discussions, let's conclude that the mechanical method of cell disruption is more suitable to recover the intracellular Ectoine at industrial scale.