Question

Control method	Type (physical/ chemical/ mechanical	Cellular target (whole cell/ cell wall/ ribosomes/etc)	Mode of treatment (sterilization / disinfection / etc)	Objects used on
Direct flame/ Incineration	PHYSICAL AGENT
Halogens -cholrine -iodine
Boiling water	PHYSICAL AGENT
Alcohol	CHEMICAL AGENT	MOST BACTERIA, VIRUSES, FUNGI
UV light
Autoclave
Pasteurization
Phenol
Cold temperatures
Heavy metals
Filtration
Acids
X-rays or ionizing radiation
Fomaldehyde
Hydrogen peroxide
Soap and detergent

Answer 1

Answere :

Direct flame/ incineration : Physical agent, kill vegetative form of bacteria but not endospore, Sterilization, nonflammable material such as loops, scissor etc.

Halogen-Chlorine and iodine : Chemical agent, oxidizing cell constituents and kill whole cells, disinfactant, disinfacting water and apply on injury area.

Boiling water : Physical agent, denature proteins and kill whole cell, sterilization, cooking purpose and prepration of media

Alcohol : Chemical agent, solublize lipids and denature proteins, Disinfactant, Disinfacting hand and apply on laboratory surfaces.

UV light : Physical agent, Kill whole cell by forming thymine dimer, sterilization, surface sterilization of equipements.

Autoclave : Physical agent, kill whole cell by denaturing protein, sterilization, sterilization of microbiological media, heat-stable medical equipement.

Pasteurization : Physical agent, denature protein and alter mrmbrane, sterilization, prevent spoilage of food, milk, juice etc.

Phenol : Chemical agent, denaturation of protein, disinfactant, used on laboraratory equipements..

Cold temprature : Physical agent, stop metabolism and may kill whole cell, sterilization, long-term storage of food, culture etc.

Heavy metals :Chemical agents, coagulatte and denature protein, disinfactant, used for sterilizing medical equipements.

Filteration : Physical agent, isolate whole bacterial cell, sterilization, Sterilization of chemicals and media.

X ray or ionizing radiaton : Physical agent, Alter molecular structure and introduce double stranded break in DNA, sterilization, used for sterilization of heat sensitive material.

Formaldehyde : Chemical agent, alkylating proteins, disinfactant, sterilizing laboratory rooms.

Hydrogen peroxide : Chemical agent, denaturation of membrane lipids, disinfactant, apply on laboratory objects.

Soaps and detergents : Chemical agent, interact with plasma membrane and kill whole cell, disinfactant, disinfacting and sanitizing medical instruments.