In: Biology
Structure and function of
transthyretin
mucin 2
catalase
calmodulin
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Transthyretin is a transport protein in the
serum and cerebrospinal fluid that carries the thyroid hormone
thyroxine (T4) and retinol-binding protein-bound to retinol.
Structure - TTR is a 55kDa homotetramer with a dimer of dimers
quaternary structure that is synthesized in the liver, choroid
plexus, and retinal pigment epithelium for secretion into the
bloodstream, cerebrospinal fluid, and the eye, respectively. Each
monomer is a 127-residue polypeptide rich in beta-sheet structure.
Association of two monomers via their edge beta-strands forms an
extended beta-sandwich.
Mucin 2 gene encodes a member of the mucin
protein family. The protein encoded by this gene, also called mucin
2, is secreted onto mucosal surfaces. Mucin 2 is particularly
prominent in the gut where it is secreted from goblet cells in the
epithelial lining into the lumen of the large intestine. There,
mucin 2, along with small amounts of related-mucin proteins,
polymerizes into a gel of which 80% by weight is oligosaccharide
side-chains that are added as post-translational modifications to
the mucin proteins.
Structure - The mucin 2 protein features a central domain
containing tandem repeats rich in threonine and proline that varies
between 50 and 115 copies in different individuals.
Catalase - Hydrogen peroxide is a harmful
byproduct of many normal metabolic processes; to prevent damage to
cells and tissues, it must be quickly converted into other, less
dangerous substances. To this end, catalase is frequently used by
cells to rapidly catalyze the decomposition of hydrogen peroxide
into less-reactive gaseous oxygen and water molecules.
Structure - Human catalase forms a tetramer composed of four
subunits, each of which can be conceptually divided into four
domains. The extensive core of each subunit is generated by an
eight-stranded antiparallel b-barrel (b1-8), with nearest neighbor
connectivity capped by b-barrel loops on one side and a9 loops on
the other. A helical domain at one face of the b-barrel is composed
of four C-terminal helices (a16, a17, a18, and a19) and four
helices derived from residues between b4 and b5 (a4, a5, a6, and
a7).
Calmodulin - The general function of CaM is to
bind calcium ions and then bind a target
protein, affecting its activity. The binding of
calcium is achieved by the 4 EF-hand domains. A
basic EF hand consists of two perpendicular alpha helices with a
12-residue loop region between them.
Structure - Calmodulin is a small, highly conserved protein that is
148 amino acids long. The protein has two approximately symmetrical
globular domains each containing a pair of EF-hand motifs (the N-
and C-domain) separated by a flexible linker region for a total of
four Ca2+ binding sites.