A scientist mutates eIF-2 to eliminate its GTP hydrolysis capability. How would this mutated form of...

A scientist mutates eIF-2 to eliminate its GTP hydrolysis capability. How would this mutated form of eIF-2 alter translation?

Expert Solution

Answer-

According to the given question-

We know that Transcription occur inside the nucleus in eukaryotes and after that RNA molecule transported from nucleus to cytoplasm where the information present in mRNA are translated into protein. The translation is Controlled by the RNA molecule. Translation starts when translation initiation complex formation takes place.

When met-tRNAi binds to small subunit or 40S of ribosome the initiation started. A protein called eukaryotic initiation factor-2 or eIF-2 is a type of protein initiation factor, brought tRNA to 40S ribosome. eIF-2 protein first binds to guanosine triphosphate or GTP molecule and after that tRNA-eIF2-GTP complex attaches to 40S ribosome subunit. Several initiation factors have the capability to recognize mRNA 5′ cap and some proteins bind to poly-A tail mRNA to form a loop like structure. eIF4F or cap-binding protein then brings both mRNA complex and tRNA-eIF2-GTP- 40S ribosome complex together after that this complex scan mRNA to find AUG or start codon. after that the initiator tRNA anticodon and AUG aligned causing GTP hydrolysis as well as release of initiation factors and large subunit of ribosome i.e. 60S ribosomal attaches to translation complex.

eIF-2 binding with RNA is regulated by phosphorylation after phosphorylation it is unable to bind with GTP. sue to which initiation complex is unable to form correctly and this effect the translation because large ribosomal subunit or 60S ribosomal unable to bind with the mRNA transcripts. but when eIF-2 is unphosphorylated this causes formation of translation initiation complex and leads to normal translation.

gladiator answered 2 years ago

Imagine that Arf1 protein was mutated so that it could not hydrolyze GTP, regardless of its...

Imagine that Arf1 protein was mutated so that it could not hydrolyze GTP, regardless of its binding partners. Would you expect COPI-coated vesicles to form normally? How would you expect transport mediated by COPI-coated vesicles to be affected? If this were the only form of Arf1 in a cell, would you expect it to be lethal? Briefly explain your answers.

Explain how microtubules polymerize from a centrosome using GTP hydrolysis.

Summarize (in 4 or more sentences, in your own words) – the structure of a microtubule and its polarity. Explain how microtubules polymerize from a centrosome using GTP hydrolysis. Complete the following table: Kinesin Dynein Structure Function(s) in a cell Direction that this motor protein moves *Highlight or use a different text color for similarities shared between kinesin and dynein. Complete the following table: Cilia Flagella Structures Function(s) Types of movements, and how these occur *Highlight or use different text...

1.When GTP hydrolysis is inhibited in the translation initiation, what would be the most possible outcome?...

1.When GTP hydrolysis is inhibited in the translation initiation, what would be the most possible outcome? Select one: A. IF1 cannot associate with 30S ribosomal subunit. B. The binding affinity of IF2 with initiator tRNA would be stronger. C. Premature binding of 50S subunit to 30S subunit. D. IF3 cannot bind to 30S subunit because the binding requires energy. E. Poor binding occurs between the first aminoacyl-tRNA and mRNA. 2. The sequence of a DNA template fragment encoding for protein...

How would introducing a non-hydrolyzable analog of ATP or GTP impact the cell? In other words,...

How would introducing a non-hydrolyzable analog of ATP or GTP impact the cell? In other words, if the ATP and GTP cannot be hydrolyzed, what specific cell processes we have discussed in class would be impacted? Have 4 different examples.

a) How does a depreciation/devaluation of a nation’s currency operate to eliminate a deficit in its...

a) How does a depreciation/devaluation of a nation’s currency operate to eliminate a deficit in its current account? (15 points) b) How do you calculate that a particular currency is undervalued? Explain your answer with a numeric example. (15 points) c) Explain the process how balance of payments deficit is eliminated in the long-run without government intervention both in the fixed (Central Bank keeping exchange rate fixed) and in the flexible exchange rates system. (20 points)

How would you respond to this post? In its simplistic form productivity equals output divided by...

How would you respond to this post? In its simplistic form productivity equals output divided by inputs(Vonderembse (2013). However, when it some to productivity it is very difficult to increase productivity with out negatively effecting quality. For example, if on an assembly line you want to increase the number of products produced per hour you can accomplish this by speeding up the assembler line. However, if you speed up the line there are more likely to be more defects in...

In the space of 5 x 5 x 2 cm3 how would you form an inductor...

In the space of 5 x 5 x 2 cm3 how would you form an inductor based on toroid geometry? Show all calculations and draw figures to justify. If half of the core of inductor is filled with some iron core of relative permeability of 4000, what will be the difference of stored energy for 2A current?

How would you, as a job interviewer analyze a candidate`s potentials, capability, dedication, attention to details?...

How would you, as a job interviewer analyze a candidate`s potentials, capability, dedication, attention to details? I think these are factors that should be included during the interview and discussed with professional references. It is easy for someone to stretch the truth, so I think it would be difficult to rely on the self-evaluation of the interviewee. What other sources would you use to learn more about a job applicant?

1.define enterprise architecture as a capability in organisations 2. restate business architecture and how to support...

1.define enterprise architecture as a capability in organisations 2. restate business architecture and how to support the business to deliver strategic business outcomes 3. Demonstrate an understanding of the components of the CSVLOD Model of Enterprise Architecture: considerations, standards, visions, landscapes, outlines and designs. 5. Review and evaluate how Governments’ use enterprise architecture to deliver citizen services 6. evaluate and investigate key trends impacting organisations today and how enterprise architects respond to these trends. can you make a essay including...

2. A scientist wants to turn a mitotic behaving cell into a meiosis behaving cell. How...

2. A scientist wants to turn a mitotic behaving cell into a meiosis behaving cell. How would they make that occur?

Question