The separated samples will be analyzed using IR and HPLC. Write four full paragraphs (at least...

The separated samples will be analyzed using IR and HPLC. Write four full paragraphs (at least 450 words) on HPLC separations and IR spectroscopy. Explain what kinds of samples are analyzed and how each instrument analyzes the sample. Describe if it is possible for each to distinguish enantiomers and how it does. Give details as to how both are used forensically.

Expert Solution

Ans:- HPLC is a separation technique in which the small amout of liquid is injected in a tube packed with tiny particles.These particles are moved in the tube with the help of particles are moved in the tube with the help of a liquid through the colum uder high pressure.The samples which can be separated by this technique are polymers, sodium chloride etc.

The HPLC can analyse the individual molecules by the time it takes for that individual compoud to move from the stationary phase to liquid phase after injection.Then we can determine the peak length of the compound that is injected and measures the concentration.Yes it is possible for HPLC to distinguish enantiomers.It collects the trace sample of the compound and calculate its purity and place it in thew HPLC to study its chirality.

HPLC is used forensically as it can detect the presence of pharmaseutical drugs,identification of tissue sample as we know HPLC technique is useful for non volatile sample and it can detect very small traces of a compound so it can be used in forensic.

IR spectroscopy is a separation technique in which a infrared spectra is obtained by passing infrared radiation through a sample and calculating the amount of energy absorbed by the sample in that particular energy.Basically the sample of HCl ,NaCl are separated by this technique where the infrared radiations are passed through the sample and there vibration are calculated.Yes it can distinguish enantiomers as the IR spectroscopic device has a optical pipe which determines the chirality of the compound.It is used forensically as it can determine the fingerprint by absorbing the infrared radiation of the sample present in human body.

queen_honey_blossom answered 2 years ago

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