In: Biology
Which of the following is a type of genetic modification? Select ALL that apply.
gene editing |
||
transformation |
||
hybridization and artificial selection |
||
Mutagenesis |
**3.Which of the following are components of the disarmed plasmid used to create transformant plants? Select ALL that apply.
Origin of replication |
||
Virulence region |
||
T region - in the transformation vector, not the disarmed plasmid |
||
selectable marker |
||
genes for conjugative transfer |
||
lacZ |
1. Genome editing, transformation, mutangenesis
All these listed processes are responsible for changing the DNA sequence.
Genome editing and mutagenesis are targeted approach for the changing the sequence of DNA. They do not involve incorporation of any external gene.
The gene is already present in an organism is changed in order to restore it to wild type or give rise to a new gene.
2. Origin of replication, T region, selective marker, lacZ
Origin of replication is a sequence which will be responsible for the replication of plasmid inside the bacterial cell.
Transfer region is important for the transfer of foreign gene into the plasmid.
Selectable marker is used for the selection of transformed bacteria against non Transformed bacteria.
LacZ gene encodes for beta galactosidase enzyme which is also a type of selectable marker and the principle is based upon blue white screening.
Please rate high.