In: Biology
Que .
When phage infect a bacterial cell, they typically have a temporal program of gene expression, i.e., they express particular genes at particular times. What are two regulatory mechanisms phage use to control their gene expression?
Please state clearly each mechanism and provide detail explanation.
A bacteriophage can use two mechanisms for its host cell
a) In the lytic
cycle process the phage attack and captures bacteria and
produces the progeny/ multiple copies of phage in large number.
b) The next method is lysogenic pathway in which the phage chromosome are inserted into host cell and they replicate within the host cell (bacterial cell)
a) Lytic cycle
1. Attaching phase in this virus attach itself to the E.coli
2. Insertion phase in this virus will inject either this DNA or RNA
into the E.coli cell/host
3. Synthesis phase in this the virus DNA or RNA will destroy the
host/ E.coli DNA and start to produce copies of their own genetic
material
4. Growing phase in this stage new viruses are formed and the virus
DNA and the capsids are made new using host (E.coli) DNA
5. The releasing phase in this stage the new viruses formed will be
released and leave the host cell (E.coli) and then capture new
cells or host.
b) Lysogenic cycle
The actual mechanism start after attaching phase, insertion
phase, virus remain inside the host cell(E.coli) without
replication and it eventually destroy the host DNA .This is latent
stage. If there is requirement of new viruses any time the original
virus may start the lytic cycle.
Example:
Herpes simplex virus