Question

Bacterial transformation allows a gene from another organism to be put into a bacterial cell. This would allow the bacterial cell to produce the protein that the gene codes for. Several steps are needed to transform bacteria; put the following steps in the correct order.

1. Add a bacterial promoter and terminator, and add sequences that will be cut by a specific restriction enzyme to the ends of the DNA piece.

2.Cut the cDNA and plasmid with the same restriction enzyme to create sticky ends.

3. Extract mRNA from the eukaryotic cell that expresses the gene.

4. Insert the plasmid carrying the gene from the other organism into the bacterial cell.

5. Produce double-stranded cDNA for the gene of interest using reverse transcriptase.

Answer 1

Ans -

3.)Extract mRNA from the eukaryotic cell that express the gene.

5.) Produce double stranded cDNA for the gene of interest using reverse transcriptase.

1.) Add a bacterial promoter and terminator ,and add sequences that will be cut by a specific restriction enzyme to the ends of the DNA piece.

2.) Cut the cDNA and  plasmid with the same restriction enzyme to create sticky ends.

Allow them to ligate and form a recombinant plasmid , having ability to replicate and express the gene of interest within the bacterial system.

4.) Insert the plasmid carrying the gene from the other organism into the bacterial cell.

so the correct order is 3 , 5 , 1, 2 , 4

Hope answer will be helpful to you ?good luck.