Question

1. TEMPERATURE EFFECTS ON CATALASE

• Results from the temperature experiment—see pg 246, 251 in lab manual):

Table 3: Temperature Effects on Catalase (pg 251 in lab manual):

Time (sec)	Tubes 2 & 3 00C	Tubes 4 & 5 220C	Tubes 6 & 7 370C	Tubes 8 & 9 480C	Tubes 10 & 11 1000C
20	0.096	0.136	0.137	0.124	0
40	0.164	0.206	0.245	0.226	0
60	0.226	0.275	0.339	0.313	0
80	0.284	0.337	0.413	0.379	0
100	0.335	0.38	0.466	0.452	0
120	0.378	0.412	0.495	0.497	0

Follow the same procedure described for enzyme availability to plot the five data series on the same graph (again, scatter plot). Record the reaction rates in table 4 below.

Answer 1

Figure 1 show scatter plot graph between Change in concentration vs Change in Time

Tubes 2 and 3, 4 and 5, 6 and 7, 8 and 9 shows moderate positive linear corelation/relationship.

Tubes 10 and 11 shows linear constant relationship.

Reaction rates [ Change in concentration of concentration / Change in Time ] of :

Tubes 2 and 3 = (0.378 - 0.096) / (120 - 20) = 0.00282 conc/secs

Tubes 4 and 5 = (0.412 - 0.136) / (120 - 20) = 0.00276  conc/secs

Tubes 6 and 7 = (0.495 - 0.137) / (120 - 20) = 0.00358  conc/secs

Tubes 8 and 9 = (0.497 - 0.124)  / (120 - 20) = 0.00373  conc/secs

Tubes 10 and 11 = ( 0 - 0) / (120 - 20) = 0  conc/secs