Question

ENZYMES DATA ANALYSIS WORKSHEET

Results:

 Table 5.1 - Catalase Activity

Tube	# Contents	Bubble Column Height (mm)
1	catalase/H2O2	27mm
2	dH2O/H2O2	0mm
3	catalase/sucrose	0mm

 Table 5.2 - Effect of Temperature

Tube #	Temp. (ºC)	Bubble Column Height (mm)
1	0	16mm
2	25	14mm
3	37	4mm
4	100	1mm

 Table 5.3 - Effect of Enzyme Concentration & Time

Tube #	Amt. of Enz.	Bubble Column Height (mm) after 20 sec.	Bubble Column Height (mm) after 2 min.
1	0 ml	0	0
2	1 ml	15mm	10mm
3	3 ml	12mm	24mm

 Table 5.4 - Effect of pH

Tube #	pH	Bubble Column Height (mm)
1	3	4mm
2	6.6	0
3	11	0

EFFECT OF pH:

1. Bubble height indicates the degree of enzyme activity. Based on the results of your experiment, which tube

had the highest bubble column height? What can you conclude about this pH?

2. Explain the results of the other two tubes. What is your conclusion concerning the effect of pH on enzyme

activity?

EFFECT OF ENZYME CONCENTRATION & TIME:

1. What is your conclusion concerning the effects of enzyme concentration and time on enzyme activity?

2. If unlimited time was allotted, would the results be the same in all tubes? Explain your answer.

ERRORS:

1. List two types of errors that could have occurred during this lab. Explain how they could have affected the

outcome of your experiments.

2. Were you surprised by any of the results you obtained in this lab? If so, what did you see and why do you

think it happened?

Answer 1

Effect of pH

1. Tube 1 had the highest bubble column height as the enzyme works in low pH in acidic condition not in alkaline condition which were in tube 2 and 3.

2. These tubes(2&3) have neutral and alkaline pH whereas the enzyme show its better activity at low pH.

EFFECT OF ENZYME CONCENTRATION AND TIME
1. The more the concentration of enzymes more compactly they Will be present providing more nom of active sites.More the time will result in higher no. Of product formation.
2. If unlimited time was allotted to both the tubes the results will never be the same as the concentration differ in both the tubes. The enzyme activity will ne reduced in tube 1 first than the second one.

Errors

1. On neutral pH there is no activity visualize but it should have show some bubble height.

2. At 1 ml conc. After 2 minutes bubble column height decrease by 5 but at 3 ml conc. The bubble column height doubled up from 12 to 24mm.