Question

If you add 100 ml of 50X TAE to 900 ml of water

a. What dilution did you perform?

b. Did you dilute the concentrated TAE to 1X?

c. What dilution is required to get to 1X?

d. To make 500 ml of 1X TAE Buffer, add _____ml of 50X TAE to _____ml of water

Answer 1

Question A. If we mix 100 mL of 50 X TAE with 900 of water, then the dilution will be one in 10 dilution.- The dilution factor can be calculated by dividing the total volume of solution by volume of stock. Hence 1000/100 = 10 times dilution.

Question B. No,

The concentration of the diluted solution can be calculated using the equation

N1V1 = N2V2

N1 = Strength of stock = 50 X

V1 = Volume of stock = 100 mL

N2 = Strength of diluted solution = ?

V2 = Volume of diluted solution = 1000 mL

by rewriting the equation

N2 = N1V1/V2

= 50 X 100/ 1000

= 5

The strength of the diluted solution is 5X

Question C

The 50 X stock can be diluted into 1X by diluting for 50 times

Question D

To make 500 ml of 1X TAE Buffer, add 10 ml of 50X TAE to 490ml of water

The volume of stock solution required to make a working solution can be calculated using the equation

  N1V1 = N2V2

N1 = Strength of stock = 50 X

V1 = Volume of stock = ?

N2 = Strength of diluted solution = 1 X

V2 = Volume of diluted solution = 500 mL

by rewriting the equation

V1 = N2V2/N1

= 500 X 1 /50

= 10 mL

10 mL of 50X stock solution + 490 ml of water will make 500 ml of 1X working solution.