Question

In: Biology

In the Meselson - Stahl experiment, if after the first round of replication, the DNA had...

In the Meselson - Stahl experiment, if after the first round of replication, the DNA had been denatured at high temperature before its centrifugation in a column of cesium chloride, which two models would have shown the same sedimentation pattern?

Group of answer choices

semiconservative and dispersive

conservative and dispersive

all three would have shown the same sedimentation pattern

semiconservative and conservative

none of the three would have had the same sedimentation pattern

Solutions

Expert Solution

Ans:

Semiconservative and dispersive

Explanation:

If after the first round of replication, the DNA had been denatured at high temperature before its centrifugation in a column of cesium chloride,  Semiconservative and dispersive models would have shown the same sedimentation pattern because both the models produce hybrid DNA (15N- 14N) after 1st round of repliction. If the cells grown on heavy medium (15N), are transferred to light medium (14N). After one round of replication both the model are produce hybrid DNA (15N- 14N). In conservative replication, If the  cells were initially grown on heavy medium (15N) and then transferred to light medium (14N). After one generation in conservative replication, they are produce Half heavy (15N) and half light DNA (14N) in CSCl equilibrium density gradient, no hybrid DNA are produce.


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