Trace the fate of the isotopic label from [2-13C]-alanine to glucose

Expert Solution

Alanine is converted to pyruvate via deamination (transamination).

This pyruvate enters gluconeogenesis to form glucose.

Pyruvate is converted to phosphoenol pyruvate (PEP) via oxaloacetate as an intermediate.

PEP is converted to glyceraldehyde-3-phosphate via 4 reactions.

Glyceraldehyde-3-phosphate is condensed with dihydroxyacetone phosphate by aldolase to form fructose-1,6-bisphosphate (FBP) which is then cleaved by FBPase into fructose-6-phosphate and Pi.

Fructose-6-phosphate is then converted to gluocose-6-phosphate which is then dephosphorylated by phosphatase to form glucose.

The 2nd carbon of alanine is incorporated into glucose as the 5th carbon. The radiolabelled carbon is shown in red colour (in the figure).

gladiator answered 1 year ago

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