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What is the effect of SNF and SWI binding to linker DNA ?down regulation , upregulation...

What is the effect of SNF and SWI binding to linker DNA ?down regulation , upregulation , blocked translation , expression , or transposition ? how does this system work ? PLEASE give a complete explanation not definition I understand the definiton . I am struggling with understanding how does this lead to expression .

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Expert Solution

DNA is composed of histones and DNA. The fundamental unit of chromatin is nucleosome which is 146 bp of DNA wrapped around histone octamer. The adjacent nucleosomes are joined by linker DNA. Nucleosome plus linker DNA is chromatosome. DNA is negatively charged and histones are positively charged due to which electrostatic interaction occurs between the two and chromatin remains in condensed form or transcriptionally inactive state or closed state.

For transcription to happen chromatin should decompact or decondense. This decondensation occurs by passive remodelling and by active remodelling. Passive remodelling occurs by post-translational modifications of histones especially histone acetylation and does not require ATP. Active remodelling requires ATP. SWI/SNF is an active chromatin remodelling complex. This complex requires ATP and physically slides nucleosomes to aside thus remodels chromatin. This remodelling makes the ground fertile for RNA polymerase to start transcription. Thus SWI/SNF augments chromatin decondensation due to which RNA polymerase finds its binding site on template DNA which ultimately leads to transcriptional activation

gladiator answered 1 year ago
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