Question
In: Biology
Write one type of method by in details[to test the HIV virus], this is experiment in...
Write one type of method by in details[to test the HIV virus], this is experiment in immunology course, I want method and results please
Solutions
Expert Solution
ANS- ELISA TEST-->Enzyme-linked immunosorbent assay is used for the detection of HIV virus , by measuring the concentration of an analyte (antibodies or antigens) in the sample solution.
PRINCIPLE--->It is based on the Antige-antibody complex, which is detected by chromogenic detection using the enzyme conjugated antibody.
PROCEDURE-
- Antigen/ sample solution is added on a plate.
- Addition of blocking buffer to block remaining protein-binding sites.
- Specific antibody is added to the coresponds of HIV virus.
- Suitable secondary antibody(HRPO conjugte) is also added on a plate,that recognises the primary antibody and binds to it.
- Then TMB (Tetramethylbenzidine )substrate is added which is converted into dectacable (produce colour) form by the help of HRPO(Horseradish peroxidase) enzyme.
RESULT-
- Light absorbing reader is used for capturing the light released from the sample, if result is positive dark spot is appeared on a plate but if it is negative the plate remains same as previous.
gladiator answered 2 years agoRelated Solutions
The standard HIV test detects HIV antibodies rather than the virus itself. When a person becomes...
The standard HIV test detects HIV antibodies rather than the
virus itself. When a person becomes infected with HIV, it takes a
few weeks for the HIV test to indicate a positive result. Based on
the information provided, which of the following processes occurs
in the time between infection and detection?
A) cell-mediated immunity
B) more macrophages are produced
C) more viruses are produced
D) humoral immunity
In May 2012, the FDA approved the first over-the-counter test for HIV, the virus that causes...
In May 2012, the FDA approved the first over-the-counter test
for HIV, the virus that causes AIDS. In a news story on July 3,
2012, the Associated Press reported that, “When used by
professionals, the test is shown to accurately identify both
carriers and non-carriers [of the HIV virus] 99 percent of the
time.” But a trial conducted by the company that developed the test
showed that individuals using the test on themselves at home
correctly detected HIV in those...
The enzyme HIV-1 reverse transcriptae (HIV-RT) plays a significant role for the HIV virus and is...
The enzyme HIV-1 reverse transcriptae (HIV-RT) plays a
significant role for the HIV virus and is an important drug target.
Assume a concentration [E] of 2.00 µM (i.e. 2.00 x 10-6 mol/l) for
HIV-RT. Two potential drug molecules, D1 and D2, were identified,
which form stable complexes with the HIV-RT.
The dissociation constant of the complex ED1 formed by HIV-RT
and the drug D1 is 1.00 nM (i.e. 1.00 x 10-9 mol/l). The
dissociation constant of the complex ED2 formed...
Type I and Type II errors were introduced recently. OraQuick In-Home HIV Test is a product...
Type I and Type II errors were introduced recently. OraQuick
In-Home HIV Test is a product that was approved by the FDA on July
3, 2012. According to the FDA website, its indications are for use
as an in-vitro diagnostic home-use test for HIV in oral fluid. The
product works by looking for the antibodies for the HIV virus. The
FDA Website and the labeling on the product warns that the results
of the test are not definitive and that...
Write a Junit test method that takes 2 Arrays of type Integer[], and tests whether these...
Write a Junit test method that takes 2 Arrays of type
Integer[], and tests whether these 2 Arrays are equal or not, and
also if the elements are all even numbers. Describe under what
conditions these 2 Arrays would be considered equal.
A certain virus infects one in every 400 people. A test used to detect the virus...
A certain virus infects one in every 400 people. A test used to
detect the virus in a person is positive 90% of the time if the
person has the virus and 10% of the time if the person does not
have the virus. Let A be the event "the person is infected" and B
be the event "the person tests positive."
(a) Find the probability that a person has the virus given that
they have tested positive.
(b) Find...
A certain virus infects one in every 400 people. A test used to detect the virus...
A certain virus infects one in every 400 people. A test used to
detect the virus in a person is positive 80% of the time if the
person has the virus and 8% of the time if the person does not have
the virus. (This 8% result is called a false positive.) Let A be
the event "the person is infected" and B be the event "the person
tests positive".
a) Find the probability that a person has the virus...
A certain virus infects one in every 400 people. A test used to detect the virus...
A certain virus infects one in every 400 people. A test used to
detect the virus in a person is positive 85% of the time if the
person has the virus and 5% of the time if the person does not have
the virus. (This 5% result is called a false positive). Let A be
the event "the person has the virus" and B be the event "the person
tests positive".
1) Find the probability that A person has the...
A certain virus infects one in every 150 people. A test used to detect the virus...
A certain virus infects one in every
150
people. A test used to detect the virus in a person is
positive
80%
of the time when the person has the virus and
55%
of the time when the person does not have the virus. (This
55%
result is called a false
positive.)
Let A be the event "the person is infected" and B be the
event "the person tests positive."
(a) Using Bayes' Theorem, when a person tests positive,
determine...
There are many obstacles to the development of an HIV vaccine, the complexity of the virus,...
There are many obstacles to the development of an HIV vaccine,
the complexity of the virus, its pathogenesis, and the human immune
response also make it difficult to determine when any
investigations vaccine satisfies ethical criteria for research use
in human subjects . Describe or explain the ethical dilemmas of
testing human candidate for HIV vaccines?
ADVERTISEMENT
ADVERTISEMENT
Latest Questions
- Define the term symbiosis specifically as it applies in a biological context. Compare and contrast parasitism,...
- A research team conducted a study showing that approximately 20% of all businessmen who wear ties...
- Research the internet for companies that have executed data science projects. Pick one company and describe...
- Explain two theories or frameworks for understanding how Western Europe emerged out of 1,000 years of...
- Two cannons are mounted as shown in the drawing and rigged to fire simultaneously. They are...
- What is the potential of a cell made up of Zn|Zn2+ and Cu|Cu2+ half-cells at 25o...
- Write a Java Program using the following instruction: Create the following 3 methods that calculate and...
ADVERTISEMENT