Question
In: Biology
Write one type of method by in details[to test the HIV virus], this is experiment in...
Write one type of method by in details[to test the HIV virus], this is experiment in immunology course, I want method and results please
Solutions
Expert Solution
ANS- ELISA TEST-->Enzyme-linked immunosorbent assay is used for the detection of HIV virus , by measuring the concentration of an analyte (antibodies or antigens) in the sample solution.
PRINCIPLE--->It is based on the Antige-antibody complex, which is detected by chromogenic detection using the enzyme conjugated antibody.
PROCEDURE-
- Antigen/ sample solution is added on a plate.
- Addition of blocking buffer to block remaining protein-binding sites.
- Specific antibody is added to the coresponds of HIV virus.
- Suitable secondary antibody(HRPO conjugte) is also added on a plate,that recognises the primary antibody and binds to it.
- Then TMB (Tetramethylbenzidine )substrate is added which is converted into dectacable (produce colour) form by the help of HRPO(Horseradish peroxidase) enzyme.
RESULT-
- Light absorbing reader is used for capturing the light released from the sample, if result is positive dark spot is appeared on a plate but if it is negative the plate remains same as previous.
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