In: Biology
250mM Tris-HCl stock solution |
400mM KCl stock solution |
20mM MgCl2 stock solution |
15mM dNTP stock solution |
10uM Forward primer stock solution |
10uM Reverse primer stock solution |
10 U/uL Taq polymerase |
4 ng/uL Template DNA |
Perform the necessary calculations to determine the volume of each component needed to achieve the below specified concentrations per reaction. When performing your calculations, be sure that you will not be required to pipette a volume less than 0.5uL, for the greatest accuracy. Use nuclease-free water to bring the total reaction volume to 25uL.