Question

4a. You want to test the hypothesis that the microbiome of a milk-fed infant is different from that of a formulafed
infant. Describe how you would test this hypothesis.
4b. Assuming you do see a difference in your experiment, how would you then test whether the distinct
microbiomes have any health benefit for a developing immune response?

Answer both questions thoroughly and completely

Answer 1

4a.Answer:

Null Hypothesis : there is no significant difference in immune respone or immunity between a microbiome of a milk fed children and a formulated milk fed children.

Alternative hypothesis : there is a difference.

This test could be conducted using Chi square test.

1) take two groups of children each with at least 30 children.

2) Group A fed with formulated milk and group B with microbiome fed milk.

3) do a boold test after a month of all the child and check how many children have high antibibodies and hemoglobin in their blood.

4)apply Chi square formula (O-E) ^2/E formula and calculate the Chi square value.

5) compare it with the table value if it is less than the table value the null hypothesis is rejected.

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Check for immunological test for the microbe.

There should not be any allergic test response in the presence of the microbe in the blood.

Immune response and the number of antibodies should have increased intge bolle for those children who have eaten microbiome of milk.

There ESR erythrocytes sedimentation rate could be checked

to conclude that they have got a good immune system.