Create a chart that compares the following methods of DNA extraction: Phenol-Chloroform, Chelex, silica-based, and differential....

Create a chart that compares the following methods of DNA extraction: Phenol-Chloroform, Chelex, silica-based, and differential. Include a very short descriptions of each, advantages and disadvantages, and special instances where they may be used.

Expert Solution

The various differences, advantages, disadvantages and usability of DNA-extraction methods can be as discussed below:

Method	Description	Advantage	Disadvantage	Usability
Phenol-chloroform	Based upon differential solubility of different bio-molecules in liquid-liquid interphase	Easy to perform, quick and does not require expensive chemicals	Low yields, DNA degradation is subjected to handling errors, cross-contamination with RNA, Proteins	Commonly used in research labs for massive extraction
Chelex	Based upon chelation of bio-molecule on a solid-liquid interface containing chelating agent in ion-exchange chromatography module	Cheap, easy and quick.	Required expertise in handling and monitoring quality and quantityt of elute, high susceptibility of chelex to DNases	Used in labs and colleges frequently
Silica-based	Based upon separation of DNA from the sample from a solid-liquid interface by capturing DNA in silica gels by micro-absorption	High quality DNA with purity and integrity is obtained. User friendly interface can be created by binding these micropore silica on chips	Silica gel columns cost revenue to the researchers	Very high purity of DNA from a variety of samples can be obtained
Differential	Isolation of DNA from different samples is performed by differential lysis procedures	DNA from different samples can be simultaneously extracted without inter-mixing	Expert handling and observational efficiency are explicitly required	DNA can be isolated from different sources by expertise in multiplexing the protocols

gladiator answered 2 years ago

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