Students collected Beta-fructosidase enzyme activity data in the
presence and absence of an unknown inhibitor. P-nitrophenol was
used as a read out for enzyme activity. Quantity of product was
determined from the absorbance of each sample after 5 minutes of
reaction. Using the information, complete the table below and
create a properly labeled Lineweaver burke plot with the
appropriate axes and best fit lines for the data, in excel (graph
is worth 6pts). Using this plot, calculate the approximate Vmax...
In a study of heart surgery, one issue was the effect of
drugs called beta-blockers on the pulse
rate of patients during surgery. The available subjects
were divided at random into two groups of
40 patients each. One group received a beta-blocker; the
other group received a placebo. The
pulse rate of each patient at a critical point during
the operation was recorded. The treatment
group had a mean pulse rate of 57 and standard deviation
7. For the control...
In a study of heart surgery, one issue was the effect of drugs
called beta-blockers on the pulse rate of patients during surgery.
The available subjects were divided at random into two groups of 30
patients each. One group received a beta-blocker; the other, a
placebo. The pulse rate of each patient at a critical point during
the operation was recorded. The treatment group had mean 65.2 and
standard deviation 7.8. For the control group, the mean was 70.3
and...
The enzyme is studied in the presence of a different inhibitor
(inhibitor B). In this case, two different concentrations of
inhibitor are used. Data are as follows:
a) Determine the apparent Vmax at each inhibitor
concentration.
b) Determine the apparent KM at each inhibitor
concentration.
c) Estimate KI from these data. [Hint: Calculate the
KI at 3 mM and at 5 mM; and then take the average of the
two KI values.]
v[(mmol/L)min−1]
[S](mmol/L)
No inhibitor
3 mM inhibitor B...
Compare and contrast characteristics of the four categories of
autonomic nervous system drugs. This response should be at least
250 words in length using AP format please. Thank you