Question

How can we explain the QF-PCR prenatal diagnostic technique procedure in detail with amniocentesis?

Answer 1

QF-PCR stands for Quantitative Fluorescence-Polymerase Chain Reaction.It amplifies specific regions of DNA and quantifies the amount of DNA present in those regions.
It helps in pre natal diagnosis to check aneuploidies, abnormalities, neural tube defects, and genetic disorders.
The standard karyotype analysis signifies the study of the number and structure of the 23 chromosome pairs. The most common chromosome anomalies, those which increase in frequency with maternal age and are related to non-disjunctional errors, result in an extra copy or loss of one chromosome, either in all cells or as a mosaic state, and are easily detected but these also have 0.1% error chances.
Procedure.
1. Genomic DNA was extracted from CV(chronic villi) samples and whole blood using the standard extraction procedure.
2.For AF(amniotic fluid) DNA extraction was performed using the Instagene Matrix, and was later on centrifuged.
3.QF-PCR was performed using a set of STR (short tandem repeats) markers for chromosomes 13, 18, 21, X, and Y.
4.  primers were fluorescently labeled with different fluorochromes and PCR(polymerase chain reaction) products were analysed.
5. A peak area curve was llater used to determines whether the sapmle had any chromosomal anomaly or not,   presence of a single peak was considered uninformative and a minimum of two concordant informative markers was required to give a result, Therefore, qF PCR can give results in amniocentesis with bwttter accuracy than conventionall method.