You are a researcher working in a molecular laboratory. One day,
you need to run a batch of PCRs. You assembled the reagents
including Taq polymerase, made the reaction mixtures, and
ran them in a thermocycler.
After the PCR program ran to completion, you performed a gel
electrophoresis to check the quality of the PCR product. To your
dismay, all reactions appeared to have failed! Nothing showed up on
the gel.
You reviewed the thermocycler’s log, which displayed the
following...