outline the operon theory, including the function cis-acting and trans -acting elements, using the lac operon...

outline the operon theory, including the function cis-acting and trans -acting elements, using the lac operon as an example

Solutions

Expert Solution

The structure of lac operon

In the presence of lactose:

In the presence of lactose the repressor binds to lactose and fails to bind to operator, so, the RNA polymease move over the structural genes and the enzymes will be synthesized.

In the absence of lactose:

In the absece of lactose the repressor binds to operator and blocks the way to RNA polymerase, so, the enzyme synthesis will be inhibited.

Find the below table for elemented and its actions of lac operon...

Gene/element	Function	Mode of control
ß-gal (lacZ)
lacZ+	Normal gene	-
lacZ-	Affects the structure of enzyme - nonfunctional	-
Permease (lacY)
lacY+	Normal gene	-
lacY-	Affects the structure of enzyme - nonfunctional	-
Trancetylase (lacA)
lacA+	Normal gene	-
lacA-	Affects the structure of enzyme - nonfunctional	-
Operator (lacO)
lacO+	Normal operator	Cis
lacOc	Constitutive operator, repressor cannot even super repressor also cannot bind to Constitutive operator	Cis
Promoter (lacP)
lacP+	Normal promoter	Cis
lacP-	RNA polymerase cannot bind	Cis
Repressor (lacI)
lacI+	Normal repressor	Trans
lacI-	Repressor is not produced	Trans
lacIs	Superrepressor, it cannot be inactivated by inducer	Trans

gladiator answered 1 year ago

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