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outline the operon theory, including the function cis-acting and trans -acting elements, using the lac operon...

outline the operon theory, including the function cis-acting and trans -acting elements, using the lac operon as an example

Solutions

Expert Solution

The structure of lac operon

In the presence of lactose:

In the presence of lactose the repressor binds to lactose and fails to bind to operator, so, the RNA polymease move over the structural genes and the enzymes will be synthesized.

In the absence of lactose:

In the absece of lactose the repressor binds to operator and blocks the way to RNA polymerase, so, the enzyme synthesis will be inhibited.

Find the below table for elemented and its actions of lac operon...

Gene/element

Function

Mode of control

ß-gal (lacZ)

lacZ+

Normal gene

-

lacZ-

Affects the structure of enzyme - nonfunctional

-

Permease (lacY)

lacY+

Normal gene

-

lacY-

Affects the structure of enzyme - nonfunctional

-

Trancetylase (lacA)

lacA+

Normal gene

-

lacA-

Affects the structure of enzyme - nonfunctional

-

Operator (lacO)

lacO+

Normal operator

Cis

lacOc

Constitutive operator, repressor cannot even super repressor also cannot bind to Constitutive operator

Cis

Promoter (lacP)

lacP+

Normal promoter

Cis

lacP-

RNA polymerase cannot bind

Cis

Repressor (lacI)

lacI+

Normal repressor

Trans

lacI-

Repressor is not produced

Trans

lacIs

Superrepressor, it cannot be inactivated by inducer

Trans

gladiator answered 2 years ago
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