Discuss eukaryotic gene regulation including the cis-acting elements and the major groups of trans-acting factors.
Discuss eukaryotic gene regulation including the cis-acting elements and the major groups of trans-acting factors. Be sure to include the relative distances from the gene and the sequence characteristics of the major elements.
Describe the difference between cis-acting and trans-acting elements. Also list whether the following elements are cis- or trans-acting: A) β-galactosidase B) Operator site C) LacI repressor protein D) Lactose E) Permease F) Promoter site
Consider the lac operon in E. coli.
a. List the cis-acting elements and the trans-acting factors
involved in the regulation of its expression.
b. Indicate which Transacting factors interact with which
cis-elements.
c. Describe what the factors do when they are bound to their
respective cis–elements.
d. Indicate the inducer in this system and how it works.
For each of the following, indicate if it is cis-acting or
trans-acting
A regulatory gene that codes for a transcription factor
_______________
A promoter _______________
An operator _________________
An enhancer _________________
1. Transcriptional regulation in eukaryotes is controlled by
both cis- and trans-acting elements. The following structure was
isolated from honey bees. It shows such elements as well as parts
of a gene body indicated with relative positions. The arrow denotes
the transcription start site (the drawing is not in scale).
Figure 1
A) Indicate what the elements or segments, number 1, 2, 3 and 4,
are most likely to stand for.
B) Which numbered segments can interact with trans-acting
factors?...
Describe the difference between cis-acting and trans-acting
elements. Also list whether the following elements are cis- or
trans-acting:
A) Beta-galactosidase
B) Operator site
C) LacI repressor protein
D) Lactose
E) Permease
F) Promoter site
Describe the structure of a typical eukaryotic gene, including only the cis-acting factors that are involved in transcription, pre-mRNA processing, and translation. Discuss which cis-acting factors are present in the gene (DNA), the pre-mRNA, and the mature mRNA