A cis-acting mutation that leads to MINIMAL expression of the lac operon would most likely be...

A cis-acting mutation that leads to MINIMAL expression of the lac operon would most likely be in the CAP binding site. Is this true or false, and why? Thanks!

Expert Solution

Answer: False -A cis-dominant mutation, is likely to be the CAP binding site Cis-acting elements are DNA sequences in the vicinity of the structural portion of a gene, which are required for gene expression. In general, cis-acting mutations are in DNA sequences that function as binding sites for proteins that control the expression of nearby genes. Mutations in a target DNA site reveal that such a site is cis-acting; that is, the target site regulates the expression of an adjacent transcription unit on the same DNA molecule. In contrast, mutations in the gene encoding an activator or repressor protein reveal that this protein is trans-acting; that is, it can act on any copy of the target DNA site in the cell.

Catabolite activator protein (CAP; also known as cAMP receptor protein, CRP) is a trans-acting transcriptional activator that exists as a homodimer in solution. Catabolyte repression is the system used by bacteria to turn off lac and other glucose-substitute operons in the presence of glucose

cAMP changes the conformation of CAP, allowing it to bind to DNA in lac operons and recruit RNA polymerase for transcription.Without cAMP, CAP cannot bind to DNA, and RNA polymerase is not recruited to the operons.

gladiator answered 1 year ago

Describe the likely effect on gene expression of a mutation in the lac operon: a) that...

Describe the likely effect on gene expression of a mutation in the lac operon: a) that delegate the operator b) that mutates the lacI gene inactivating the repressor c) that the promoter mutates in the -10 region

Please emphasize if the following are cis-acting, trans-acting or both on the lac operon. The lac...

Please emphasize if the following are cis-acting, trans-acting or both on the lac operon. The lac repressor Protein: The Operator sequence: The Operon sequence: RNA Polymerase II

outline the operon theory, including the function cis-acting and trans -acting elements, using the lac operon...

outline the operon theory, including the function cis-acting and trans -acting elements, using the lac operon as an example

Consider the lac operon in E. coli. a. List the cis-acting elements and the trans-acting factors...

Consider the lac operon in E. coli. a. List the cis-acting elements and the trans-acting factors involved in the regulation of its expression. b. Indicate which Transacting factors interact with which cis-elements. c. Describe what the factors do when they are bound to their respective cis–elements. d. Indicate the inducer in this system and how it works.

Which of the following genes of the Lac operon encode cis-acting regulatory elements?

Which of the following genes of the Lac operon encode cis-acting regulatory elements? Lacy Lacl None of these answers is correct LacA Lacz

A mutation prevents expression of the CAP protein. Will the Lac operon be expressed when lactose...

A mutation prevents expression of the CAP protein. Will the Lac operon be expressed when lactose levels are high and glucose levels are low? Explain your reasoning.

1. For the lac operon, explain what would happen and why regarding expression of the operon...

1. For the lac operon, explain what would happen and why regarding expression of the operon in each of the following scenarios. Be sure to include both positive and negative regulation in your explanation. a.) lactose is present, glucose is absent b.) lactose is absent, glucose is present c.) both lactose and glucose are present d.) lactose is present, glucose is absent, but there is a mutation to the sequence in the CAP site

Predict the level of gene expression of the lac operon, as well as the status of...

Predict the level of gene expression of the lac operon, as well as the status of the lac repressor and the CAP protein, when bacterial growth media contains the following sugars: a) no lactose or glucose, b) lactose but no glucose, c) glucose but no lactose, d) both lactose and glucose.

The necessary components for gene expression are often described as either cis-acting elements (“acting from the...

The necessary components for gene expression are often described as either cis-acting elements (“acting from the same molecule”) or trans-acting factors (“acting from a different molecule”). Cis-acting elements are typically sequences that are required for expression. Trans-acting factors are typically proteins that recognize and bind cis-acting sequences to control or facilitate gene expression. For each of the cis-acting elements below, Promotor and Start Codon,list a trans-acting factor involved in its recognition and describe the factor’s normal function

In E coli the lac operon is used to control the expression of the genes involved...

In E coli the lac operon is used to control the expression of the genes involved in the used and metabolism of lactose . describe how the genes are not expressed in the absence of lactose. describe how the genes are expressed when lactose is present both in the presence and of glucose. what would happen if there were an l- mutation? what would happen if there were an Oc mutation? which one of those two mutations is recessive ?

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