Question

In: Biology

After restriction digestion of a DNA molecule, following fragments were generated - 500 bps, 350 bps,...

After restriction digestion of a DNA molecule, following fragments were generated - 500 bps, 350 bps, 100 bps and 40 bps. If you load all these fragments on an agarose gel, this fragment_(a)___________ would move farthest and this fragment _(b)___________ would move slowest under the influence of electricity?

(a)500 bps, (b) 40 bps

(a) 40 bps, (b) 350 bps

(a) 40 bps, (b) 500 bps

(a) 100 bps, (b) 350 bps

Which of the following statements about STRs is False?

These are 2-9 base nucleotides long.

Variation in the number of repeats is used in preparing DNA profiles.

They consist of differences in DNA sequences that are only one nucleotide long.

Clusters of STRs are found widely distributed on all human chromosomes.

Which of the following statements is NOT TRUE about findings from Human Genome Project?

15 % of the DNA codes for Protein and rest 85% is non coding DNA

Human genome is made up of 3.2 billion nucleotide base pairs

Repetitive DNA makes most of the non coding DNA.

Our DNA has 20,000-25,000 genes

Which of the following is NOT a goal of Human Genome Project?

To find the location of all genes in our genomes

To compile lists of expressed genes and non expressed sequences.

To find out drugs for the treatment of genetic disorders.

To compare genes and their proteins among different species

Following are the steps of gene therapy. Pick the option from multiple choice that SHOWS the correct order of gene therapy steps:

i. Healthy cells are screened, ii. Normal genes are inserted into the removed body cells by using genetically modified viruses, iii. Cells from infected individual are removed from the body, iv. Healthy cells are returned to the body.

iii, ii, i, iv

ii, i, iii, iv

Reema was diagnosed with pancreas cancer which has now spread to her other body parts like lungs, liver etc. Which ONE of the following terms CAN'T be used to describe her cancer?

Highly likely, her cancer in pancreas started as a benign tumor

Her cancer might have started as malignant tumor

Her cancer is invasive and is spreading to other body parts.

Her cancer is a metastatic cancer

i, ii, iii, iv

iv, iii, ii, I

Solutions

Expert Solution

1) After restriction digestion of a DNA molecule, following fragments were generated - 500 bps, 350 bps, 100 bps and 40 bps. If you load all these fragments on an agarose gel, this fragment_(a)___________ would move farthest and this fragment _(b)___________ would move slowest under the influence of electricity?

The answer is

(a) 40 bps, (b) 500 bps

In gel electrophoresis the molecules move based on molecular weight and charge. So small molecules will move faster than larger molecules

2) Which of the following statements about STRs is False?

The answer is

They consist of differences in DNA sequences that are only one nucleotide long.

3) Which of the following statements is NOT TRUE about findings from Human Genome Project?

The answer is

15 % of the DNA codes for Protein and rest 85% is non coding DNA (ONLY 1% IS CODING )

4) Following are the steps of gene therapy. Pick the option from multiple choice that SHOWS the correct order of gene therapy steps:

The answer is iii, ii, i, iv

iii. Cells from infected individual are removed from the body,

ii. Normal genes are inserted into the removed body cells by using genetically modified viruses,

i. Healthy cells are screened,  

iv. Healthy cells are returned to the body.


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