You are preparing two liters of media for pouring LB/Carb agar plates. The final concentration of...

You are preparing two liters of media for pouring LB/Carb agar plates. The final concentration of
carbenicillin needs to be 100 μg/ml, and you have a stock carbenicillin solution that is 100
mg/ml. How much of the stock Carb solution do you need to add to the media after in cools
following autoclaving?

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Expert Solution

we use the formula C1V1 = C2V2

Where

C1 = concentration of stock solution

V1 = volume of the stock needed

C2 = concentration of working solution

V2 = total volume of solution prepared

So, C1 = 100mg/ml or 100*10^3 microgram/ml

V1 = x

C2 = 100 microgram/ml

V2 = 2*10^3 ml or 2 lt

By using the formula

C1V1 = C2V2

100*10^3 * x = 100 * 2* 10^3

x = 2ml

We need 2 ml of stock solution i,e carbenicillin (100mg/ml) in 2lt of lb-agar.

So, after autoclaving when the media cools down (not solidify) add 2ml of carbenicillin stock solution in it, mix well then pour this to make lb/carb agar plates.

gladiator answered 1 year ago

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