Question

True or false?

The bicinchoninic acid (BCA) assay can measure the amount of protein in your cell extract.

Tubulin is one of the main components of the extracellular matrix (ECM).

Myosin is a component of the thick filaments in the sarcomeres of skeletal muscles.

The positive end of actin is closest to the Z-line.

Answer 1

1. True

The Bicinchoninic acid assay (BCA) is a process for determing the total concentration of the protein in a solution. The total amount of protein is quantified by the color change of the sample solution from green to purple in proportion to protein concentration, which is further measured by  colorimetric techniques

2. False

The composition of Extracellular matrix consists of proteoglycans, water, minerals, and fibrous proteins, where proteoglycans is composed of protein called glycosaminoglycans. The Tubulin is found generally in centrosomes and spindle pole bodies, since these are the areas of most abundant microtubule nucleation

3. True

Myosin and actin are the 2 protein found in sarcomer, myosin is the thick filament, and actin, which forms the thin filament.

4. True

Actin is a thin filament, globular protein that polymerizes to form long filaments. There are 2 ends of actin filaments differ in their dynamics of subunit addition and removal, the plus end (ast dynamic; barbered end) and minus end (slow dynamic) .The plus end of the actin is attached to the Z-disc/Z-line, which includes the crosslinking protein α-actinin and the minus end is free.

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