Question 1 a) What is the significance of the N.A. number inscribed on the outer barrel...

Question 1 a) What is the significance of the N.A. number inscribed on the outer barrel of an objective? b) What is meant by depth of field? c) With an objective of a given magnification, why shouldn't one use increasingly higher magnification eyepieces to achieve higher total magnification? d) In a light microscope an object is placed 2 mm away from a lens of diameter 2 mm. The object is in air (refractive index = 1) and the wavelength of the light (green) is 520 nm. i. What is the one-half angular aperture of the objective? ii. What is the best possible resolving power of this microscope? e) Calculate the eth o iel or a resolin oer o m in a microscoe ith a final aperture of diameter 1 mm and a working distance of 20 mm. f) Why is optical microscopy not suitable for observations of fracture surfaces? g) What is the advantages of the confocal microscopy over optical microscopy?

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genius_generous answered 2 years ago

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