Question

Create a series of slides using PowerPoint or Google Slides to explain how an integral membrane protein is made, beginning with DNA and ending with the final product/location. Your slides should specify the intracellular location and the organelle(s) involved for each step. Please limit your submission to 10 slides.

Answer 1

Synthesis of Integral Membrane Protein

Synthesis of integral membrane protein occurs in Endoplasmic Reticulum.Biosynthesis of integral membrane protein involves several interrelated events:

• Targeting of the nascent chain to the Endoplasmic Reticulum
• Translocation of all necessary domains into the Endoplasmic Reticulum lumen
• Recognition & proper orientation of Transmembrane domains
• Integration of Transmembrane domains into the lipid bilayer & in some cases, formation of multimeric complexes.
• Nucleus encoded proteins begin translation into the cytosol
• secretory & integral membrane proteins have a signal sequence that is recognised by the signal recognition particle (SRP), shortly after emerging from the ribosome
• Through interactions with its receptor on the surface of the endoplasmic reticulum, SRP transfers the ribosome-nascent-chain complex to the translocon, an aqueous pore in the endoplasmic reticulum membrane responsible for translocation & integration
• At the endoplasmic reticulum, upon entering the translocon, integral membrane proteins differ from secretory proteins in that translocation stops & transmembrane domains are oriented and integrated into the bilayer.