Question

1) Explain the similarities and differences between the RBC and Dicty lanes.?

2) Explain how the Dicty-5 and Dicty-10 samples differ from each other and discuss whether this was what you expected to see.?

NOTE:

combine 5 ?l of your Dictyostelium sample and 95 ?l of 0.15N NaCl.

Dicty-5 is Dictyostelium discoideum cells.

Dicty-10 is Dictyostelium discoideum cells. ?just the value is change

Answer 1

Answer 1.

Red blood cells (RBCs), also called erythrocytes, are the most common type of blood cell and the vertebrate's principal means of delivering oxygen (O2) to the body tissuesâvia blood flow through the circulatory system.[1] RBCs take up oxygen in the lungs, or gillsof fish, and release it into tissues while squeezing through the body's capillaries.

Dictyostelium discoideum is a species of soil-living amoeba belonging to the phylum Amoebozoa, infraphylum Mycetozoa. Commonly referred to as slime mold, D. discoideum is a eukaryote that transitions from a collection of unicellular amoebae into a multicellular slug and then into a fruiting body within its lifetime.

The only similarity between RBC and Dictyostelium discoideum that they are both involved in Phagocyosis

• D. discoideum is known for eating Gram-positive, as well as Gram-negative bacteria, but some of the phagocytized bacteria, including some human pathogens, are able to live in the amoebae and exit without killing the cell. When they enter the cell, where they reside, and when they leave the cell are not known. The research is not yet conclusive but it is possible to draw a general lifecycle of D. discoideum adapted for farmer clones to better understand this symbiotic process.
• The aging red blood cell undergoes changes in its plasma membrane, making it susceptible to selective recognition by macrophages and subsequent phagocytosis in the mononuclear phagocyte system (spleen, liverand lymph nodes), thus removing old and defective cells and continually purging the blood. This process is termed eryptosis, red blood cell programmed cell death.

It has also been observed that slight differences in protein mobilities occur when the same proteins are run in different SDS-PAGE buffer systems (2). Each SDS PAGE buffer system has a different pH, which affects the charge of a protein and its binding capacity for SDS. The degree of change in protein mobility is usually small in natural proteins but is more pronounced with atypically or chemically modified proteins, such as pre-stained standards.

There will be difference in movements of Dicty 5 and dicty 10 as there is a difference in Ph in both lanes.