1) 5pts - Discuss how an E. coli origin of replication is recognized [sequences?], opened [by...

1) 5pts - Discuss how an E. coli origin of replication is recognized [sequences?], opened [by dnaA], and helicase is added – include any important sequences and their roles in the story. How is this origin licensed?

2) Discuss how promoters, enhancers and the TF proteins that interact with them are used in eukaryotic cells to control transcription of housekeeping genes versus cell specific/regulated genes? Be specific about everything’s role – what binds where and does what!

A) 4pts - Regulated cell specific genes -

B) 4pts - Housekeeping genes –

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