Complement pathways _
Explain how classical pathway, Alternative pathway, lectin pathway
and terminal pathway are activated to destroy bacterial cells?
4. Describe the appendages of bacterial cells.
a. How motility is accomplished in
bacterial cells.
b. What are pilin and the role of pili and
fimbriae.
c. Explain the structure of flagella.
1) Please explain how to transform bacterial cells with plasmid
DNA.
2) Please explain how to purify plasmid DNA from bacterial
cells.
3) How can you use PCR to clone a 2000bp gene into a plasmid
vector?
4) Please explain how a fluorescent tag can be used to visualize
protein localization in a cell.
Describe the classical pathway of complement activation in the
absence of antibodies and how it is used during an innate immune
response to clear the pathogen.
Explain the importance of immunological memory cells. Describe
in detail how and when memory cells arise and explain how they form
the basis for effective immunizations.