Question

1. What three organs or tissue types does Maximov describe as being “blood producing”?

2. Gail Martin used a teratoma formation in her paper on mouse embryonic stem cells. What is a teratoma?

3. Why is this assay relevant to her claim that she had isolated multipotent stem cells?

4. Multiple stem cell researchers used “fibroblast feeder cells” during their stem cell isolation experiments. What is a fibroblast?

5. Were the fibroblasts altered in any way, and if so, why?

6. What role did the fibroblasts provide to the co-cultured stem cells?

7. Takahashi and Yamanaka (2006) discovered that they could reprogram somatic tissues back to a “stem” state. Why did they choose to reprogramming cells from a pan-GFP mouse line? What proof did that bring to their studies?

8. What terms or phrases did you come across in Chapter 1 of the textbook that were confusing or difficult to understand?

Please Answer all questions!

Answer 1